|Minimum Order Quantity||1 Bottle|
|Packaging Details||100 GM|
|Usage/Application||Use: Recommended for detection of Escherichia coli and coliforms in water samples. The composition a|
|Packaging Size||100 GM|
|Brand||citreos fine chem|
|Reference||Atlas, R. M. (2005). Handbook of media for environmental microbiology. CRC press. 2. Baird R.B., Eat|
|Incubation||Directions: Dissolve 31.00 grams (the equivalent weight of dehydrated medium per liter) in 1000 ml d|
|State Of Matter||Solid|
Chromogenic Coliform Agar is a selective medium recommended for the simultaneous detection andrecovery of sub-lethally injured coliforms in water samples. The CCA media is composed of tryptone, yeastextract, sodium chloride, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium pyruvate,sorbitol, tryptophan, tergitol-7 and three chromogenic substrates. The three chromogenic substrates are 6-chloro-3-indoxyl β-D-galactopyranoside, 5-bromo-4-chloro-3-indoxyl- β-D-glucuronic acid cyclohexamineammonium salt, monohydrate and IPTG (Isopropyl-β-D-thiogalactopyranoside). Tryptone, sodium pyruvateand sorbitol provide nitrogenous substances, fermentable carbohydrate and other essential growth nutrientsfor the organisms. The L-Tryptophan improves the indole reaction thus increasing the revealing reliability.
Sodium dihydrogen phosphate, disodium hydrogen phosphate buffers the medium. Tergitol-7 inhibits gram-positive as well as some gram-negative bacteria other than coliforms. The enzyme ß-D-galactosidase
produced by coliforms hydrolyzes 6-chloro-3-indoxyl-β-D-galactopyranoside to form pink to red colored
colonies. The enzyme ß-D-glucuronidase produced by E.coli, cleaves 5-bromo-4chloro-3-indoxyl-β-D-glucuronic acid. Colonies of E.coli give dark blue to violet colored colonies due to cleavage of both the
chromogens. The presence of the third chromogenic IPTG enhances the color of reaction.
- Production Capacity: 100 BOTTLE
- Delivery Time: 2-3 DAYS
- Packaging Details: 100 GM hdpe bottle